Bishop et al — study overview
Resected NSCLC cases
- 102 resected NSCLCs were classified as SqCC or adenocarcinoma using traditional histologic features and immunohistochemical profiles and MiRview™ squamous
Preoperative biopsies/aspirates NSCLC cases
- 21 preoperative biopsies/aspirates that had been originally diagnosed as poorly differentiated NSCLCs
- A qRT-PCR diagnostic assay measured hsa-miR-205 expression levels to classify the carcinomas as SqCC or adenocarcinoma based solely on expression levels
- The sets of classifications were then compared
Case 87 from Bishop et al
This sample was diagnosed as a poorly differentiated NSCLC based on a preoperative fine-needle aspiration.
1. The unstained sample shows scattered discohesive and degenerating atypical cells in a background of blood
2. Using nuclear p63 immunolabeling, the sample suggests a SqCC
3. The subsequent lung resection shows an adenocarcinoma with clear-cut glandular differentiation
4. Using microRNA biomarkers, both the preoperative aspirate and resection samples were diagnostic of adenocarcinoma
Figure reprinted with permission from the American Association for Cancer Research. Figure 3 from Bishop JA, Benjamin H, Cholakh H, Chajat A, Clark DP, Westra WH. Accurate classification of non–small cell lung carcinoma using a novel microRNA-based approach. Clin Cancer Res. 2010;16(2):610-619.
